Part:BBa_K1369006

pBAD FsrA, pFsrA-GFP, RR with no recruitment

This plasmid contains FsrA, which is a response regulator used in E. coli. In our project, we were designing a two-component system, with a histidine kinase and response regulator. If there is a change in the environment that the histidine kinase sense, the response regulator will act in response to it and express GFP.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3656
  Illegal XbaI site found at 5291
  Illegal XbaI site found at 6977
  Illegal SpeI site found at 3662
  Illegal PstI site found at 8166
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3656
  Illegal NheI site found at 4906
  Illegal SpeI site found at 3662
  Illegal PstI site found at 8166
  Illegal NotI site found at 1
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3656
  Illegal BglII site found at 4929
  Illegal XhoI site found at 5679
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3656
  Illegal XbaI site found at 5291
  Illegal XbaI site found at 6977
  Illegal SpeI site found at 3662
  Illegal PstI site found at 8166
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3656
  Illegal XbaI site found at 5291
  Illegal XbaI site found at 6977
  Illegal SpeI site found at 3662
  Illegal PstI site found at 8166
  Illegal NgoMIV site found at 2275
  Illegal NgoMIV site found at 7432
  Illegal NgoMIV site found at 7715
  Illegal AgeI site found at 2435
  Illegal AgeI site found at 4690
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 6692
  Illegal BsaI site found at 6870
  Illegal BsaI site found at 8803
  Illegal BsaI.rc site found at 6528
  Illegal SapI site found at 4672
  Illegal SapI site found at 5259
  Illegal SapI site found at 7655
  Illegal SapI site found at 7865