Signalling

Part:BBa_K1369005

Designed by: Andrew Hou   Group: iGEM14_Caltech   (2014-09-11)

AgrA; P2 promoter+GFP

This sequence contains an inducible pBAD promoter regulating expression of the AgrA response regulator. Downstream of the AgrA coding sequence is a GFP reporter gene, regulated by the P2 promoter. This part goes together with part BBa_K1369003 to form the AgrC/AgrA two-component system. The figure below illustrates the circuit that should be formed by parts K1369003 and K1369005.

Diagram showing SH3 scaffold mechanism used to test the AgrC/AgrA two-component system. This part codes for the AgrA response regulator. This part also contains the P2 promoter regulating expression of super-folder GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 312
    Illegal XhoI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 73
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1874
    Illegal SapI site found at 55


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Categories
Parameters
None