Signalling

Part:BBa_K1369004

Designed by: Andrew Hou   Group: iGEM14_Caltech   (2014-09-04)

AgrA+SH3 peptide; P2 promoter+GFP

This sequence contains an inducible pBAD promoter regulating expression of the AgrA response regulator with an SH3 peptide attached to the N-terminus. Downstream of the AgrA coding sequence is a GFP reporter gene, regulated by the P2 promoter. This part goes together with BBa_K1369003 to form the AgrC/AgrA two-component system, coupled with SH3 scaffolds. The diagram below illustrates the circuit that should be formed by parts K1369003 and K1369004.


Diagram showing SH3 scaffold mechanism used to test the AgrC/AgrA two-component system. This part codes for the AgrA response regulator with an SH3 peptide attached at the N-terminus. This part also contains the P2 promoter regulating expression of super-folder GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1011
    Illegal BglII site found at 312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 73
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1946
    Illegal SapI site found at 55


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Categories
Parameters
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