Signalling
Part:BBa_K1369003
Designed by: Andrew Hou Group: iGEM14_Caltech (2014-09-04)
AgrC+4xSH3 domain
This sequence encodes a constitutively expressed AgrC histidine kinase with a 4xSH3 scaffold domain attached at the C-terminus. This part works together with part BBa_K1369004 to form the AgrC/AgrA two-component system, coupled with SH3 scaffolds. The diagram below illustrates the circuit that should be formed by parts K136903 and K1369004.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 30
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 68
Illegal BglII site found at 438
Illegal BamHI site found at 2186
Illegal XhoI site found at 2195 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1481
Illegal SapI.rc site found at 1691
Illegal SapI.rc site found at 1901
Illegal SapI.rc site found at 2111
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Categories
Parameters
None |