Signalling

Part:BBa_K1369003

Designed by: Andrew Hou   Group: iGEM14_Caltech   (2014-09-04)

AgrC+4xSH3 domain

This sequence encodes a constitutively expressed AgrC histidine kinase with a 4xSH3 scaffold domain attached at the C-terminus. This part works together with part BBa_K1369004 to form the AgrC/AgrA two-component system, coupled with SH3 scaffolds. The diagram below illustrates the circuit that should be formed by parts K136903 and K1369004.


Diagram showing SH3 scaffold mechanism used to test the AgrC/AgrA two-component system. This part codes for the AgrC histidine kinase with 4 SH3 domains attached at the C-terminus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 68
    Illegal BglII site found at 438
    Illegal BamHI site found at 2186
    Illegal XhoI site found at 2195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1481
    Illegal SapI.rc site found at 1691
    Illegal SapI.rc site found at 1901
    Illegal SapI.rc site found at 2111


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Categories
Parameters
None