Composite

Part:BBa_K1368009

Designed by: Lu Wang   Group: iGEM14_HZAU-China   (2014-09-29)


Pluxr+taRNA+T+Pluxr+CrRNA+cre

The main purpose of this part is cre protein expression.Then we add the taRNA and crRNA to reduce the leakage of the luxR promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 453
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 61
    Illegal BsaI.rc site found at 300


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Categories
Parameters
None