Composite

Part:BBa_K1368003

Designed by: liu Tian   Group: iGEM14_HZAU-China   (2014-09-29)

ptetR+lox66+lacI+plac(R0011)+c1

This part is the base composite of the oscillator.If you add the tetR protein under lambda cI regulated promoter,you can get a complete oscillator.Furthermore, because of the lox66 between the ptetR and lacI protein, you can design many different devices after rewired by insert the other lox site at different place or direction in the tetR protein under lambda cI regulated promoter. abc

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1269
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None