Composite
C5

Part:BBa_K1366104

Designed by: Eduardo Cepeda Cañedo, Mercedes Alejandra Vazquez Cantu   Group: iGEM14_Tec-Monterrey   (2014-10-10)

Apoptin expression under a CMV promoter (C5)

This construct will be used to characterize the transfection of a death effector (apoptin) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. Apoptin will be codon optimized for mammalian cell line.

In the next image it is presented the restriction digestion analysis of some biobricks:

Gel_psb1c3.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1428


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Categories
Parameters
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