Composite

Part:BBa_K1366022

Designed by: Eduardo Cepeda Cañedo   Group: iGEM14_Tec-Monterrey   (2014-10-10)


Genetic construct for msbB gen deletion

This biobrick contains the sequence homologies (50bp) to delete msbB gene (LPS moiety carrier) in E. coli with a kanamycin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gene in E. coli). The deletion of msbB gene and the replacement of that sequence with the Kan resistance are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance after the recombination with a specific recombinase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1106
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 619
    Illegal SapI.rc site found at 829


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Parameters
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