Composite

Part:BBa_K1366001

Designed by: Eduardo Cepeda Cañedo   Group: iGEM14_Tec-Monterrey   (2014-10-10)


Genetic construct for lpp gen deletion

This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1290
    Illegal XbaI site found at 64
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1291
    Illegal PstI site found at 1305
    Illegal NotI site found at 7
    Illegal NotI site found at 1298
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1291
    Illegal XbaI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal XbaI site found at 64
    Illegal SpeI site found at 1291
    Illegal PstI site found at 1305
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None