Plasmid_Backbone
Part:BBa_K1365301
Designed by: Lisa Hielkema Group: iGEM14_Groningen (2014-10-08)
pIL253-mRFP Biobrick Vector for L.lactis
This plasmid can be used to transform biobricks in L. lactis. Moreover it shuttles between L. lactis and B. subtilis. It contains mRFP Biobrick Prefix and Sufix. And it has an erythromycin resistance.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 124
Illegal suffix found at 1215 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 124
Illegal NheI site found at 1940
Illegal SpeI site found at 1216
Illegal PstI site found at 1230
Illegal NotI site found at 130
Illegal NotI site found at 1223 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 124 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 124
Illegal suffix found at 1216 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 124
Plasmid lacks a suffix.
Illegal XbaI site found at 139
Illegal SpeI site found at 1216
Illegal PstI site found at 1230
Illegal AgeI site found at 926
Illegal AgeI site found at 1038 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
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Categories
Parameters
None |