Plasmid_Backbone

Part:BBa_K1365301

Designed by: Lisa Hielkema   Group: iGEM14_Groningen   (2014-10-08)


pIL253-mRFP Biobrick Vector for L.lactis

This plasmid can be used to transform biobricks in L. lactis. Moreover it shuttles between L. lactis and B. subtilis. It contains mRFP Biobrick Prefix and Sufix. And it has an erythromycin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 124
    Illegal suffix found at 1215
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 124
    Illegal NheI site found at 1940
    Illegal SpeI site found at 1216
    Illegal PstI site found at 1230
    Illegal NotI site found at 130
    Illegal NotI site found at 1223
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 124
    Illegal suffix found at 1216
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 124
    Plasmid lacks a suffix.
    Illegal XbaI site found at 139
    Illegal SpeI site found at 1216
    Illegal PstI site found at 1230
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1038
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None