Reporter

Part:BBa_K1365020

Designed by: Sandra Mous   Group: iGEM14_Groningen   (2014-09-24)

sfGFP(Bs)

Usage and Biology

This gene codes for the sfGFP(Bs) protein, an optimized fluorescent reporter. This sfGFP was originally optimized for Bacillus subtilis. When it was tested in different species it showed high fluorescence in Lactococcus lactis. The sfGFP(Bs) has a very high performance in L. lactis compared to other GFP proteins, and also works really well in other bacteria like B. subtilis, Escherischia coli and Streptococcus pneumoniae.

Sfgfpbs.png


Sequence and features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Overkamp, W. et al. (2013) Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging. Appl. Environ. Microbiol. 79: 6481-6490


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