Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Composite

Part:BBa_K1364017

Designed by: Jourdan Camille   Group: iGEM14_Toulouse   (2014-10-02)

Promotor PlepA + RBS spoVG

PlepA is a constitutive promotor in Bacillus subtilis (BBa_K823002) coupled with a RBS spoVG (BBa_K143021). To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

This construction is also working with E. coli and has been verified by sequencing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//regulation
Parameters
n/aPromotor PlepA + RBS spoVG