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Reporter

Part:BBa_K1364016

Designed by: Jourdan Camille   Group: iGEM14_Toulouse   (2014-10-03)

PlepA + RFP

This part is designed to enable the expression of a Red Fluorescent Protein in Bacillus subtilis under the control of a constitutive promotor PlepA. This construction has been checked by sequencing and has shown to work also in E. coli.

Design
This part is composed of a constitutive promotor PlepA (K823002), spoVG RBS (K143021), the coding sequence of the RFP (E1010) and a double terminator (B0015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 738
    Illegal AgeI site found at 850
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//function/reporter/fluorescence
Parameters
n/aPlepA + RFP