Generator

Part:BBa_K1364008

Designed by: Laureen Mirassou   Group: iGEM14_Toulouse   (2014-10-01)

Pveg - strong RBS - Antifungal GAFP-1 - Double terminator

This expression cassette is designed for the expression and secretion of the Gastrodia anti-fungal protein (GAFP-1). GAFP-1 is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.

Design
The part is composed of a constituve promoter (K143012 Pveg), a strong RBS with the coding sequence of GAFP-1 (K1364002) and a double terminator (B0015). The coding sequence of GAFP-1 is flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
n/aPveg - strong RBS - Antifungal GAFP-1 - Double terminator