Pveg - strong RBS - Antifungal GAFP-1 - Double terminator
This expression cassette is designed for the expression and secretion of the Gastrodia anti-fungal protein (GAFP-1). GAFP-1 is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.
The part is composed of a constituve promoter (K143012 Pveg), a strong RBS with the coding sequence of GAFP-1 (K1364002) and a double terminator (B0015). The coding sequence of GAFP-1 is flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 164
- 1000COMPATIBLE WITH RFC
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