Generator

Part:BBa_K1364004

Designed by: Jourdan Camille   Group: iGEM14_Toulouse   (2014-10-03)

Pveg + N-acetylatedglucosamine based chemotaxis for Bacillus subtilis

This part is designed to enable a N-acetylatedglucosamine based chemotaxis in Bacillus subtilis.


Design
This part is composed of a constituve promoter (K143012, Pveg) and BBa_1364000 (Strong RBS K780002, open reading frame of chimeric chemotaxis protein and a double terminator B0015)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2158
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1615
    Illegal SapI.rc site found at 2251


Functionnal characterization of the BioBrick : :
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References :
K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman and G. Schoolnik. The Vibrio cholerae chitin utilization program . The National Academy of Sciences of the USA (2004).

C. Kristich and GW. Ordal.Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.

[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
n/aPveg + N-acetylatedglucosamine based chemotaxis for Bacillus subtilis