Pveg + N-acetylatedglucosamine based chemotaxis for Bacillus subtilis
This part is designed to enable a N-acetylatedglucosamine based chemotaxis in Bacillus subtilis.
This part is composed of a constituve promoter (K143012, Pveg) and BBa_1364000 (Strong RBS K780002, open reading frame of chimeric chemotaxis protein and a double terminator B0015)
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 2158
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 1615
Illegal SapI.rc site found at 2251
Functionnal characterization of the BioBrick : :
K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman and G. Schoolnik. The Vibrio cholerae chitin utilization program . The National Academy of Sciences of the USA (2004).
C. Kristich and GW. Ordal.Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.
|n/a||Pveg + N-acetylatedglucosamine based chemotaxis for Bacillus subtilis|