Part:BBa_K1364000

N-acetylatedGlucosamine based chemotaxis for Bacillus subtilis

This part is designed to enable a N-acetylatedglucosamine based chemotaxis in Bacillus subtilis.

Design :
The encoded protein is a chimera of 2 proteins:
-the methyl accepting Chemotaxis protein (McpA, http://www.uniprot.org/uniprot/P39214) from Bacillus subtilis which is required for taxis towards glucose,
-and the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from Vibrio cholerae (VCD_000306, http://www.uniprot.org/uniprot/C3NYT2). The chimeric protein contains the intracellular domains of McpA from Bacillus subtilis to enable the transduction of the signal: these domains correspond to the amino acids 1-37 and 282-610. The extracellular domain of VCD_000306 is inserted between the intracellular regions of McpA to sense N-acetylglucosamine and corresponds to the amino acids 31-310.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1913
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1370
  Illegal SapI.rc site found at 2006
  

References :
K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman and G. Schoolnik. The Vibrio cholerae chitin utilization program . The National Academy of Sciences of the USA (2004).

C. Kristich and GW. Ordal.Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.