N-acetylatedGlucosamine based chemotaxis for Bacillus subtilis
This part is designed to enable a N-acetylatedglucosamine based chemotaxis in Bacillus subtilis.
The encoded protein is a chimera of 2 proteins:
-the methyl accepting Chemotaxis protein (McpA, http://www.uniprot.org/uniprot/P39214) from Bacillus subtilis which is required for taxis towards glucose,
-and the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from Vibrio cholerae (VCD_000306, http://www.uniprot.org/uniprot/C3NYT2). The chimeric protein contains the intracellular domains of McpA from Bacillus subtilis to enable the transduction of the signal: these domains correspond to the amino acids 1-37 and 282-610. The extracellular domain of VCD_000306 is inserted between the intracellular regions of McpA to sense N-acetylglucosamine and corresponds to the amino acids 31-310.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1913
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 1370
Illegal SapI.rc site found at 2006
K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman and G. Schoolnik. The Vibrio cholerae chitin utilization program . The National Academy of Sciences of the USA (2004).
C. Kristich and GW. Ordal.Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.
|n/a||N-acetylatedGlucosamine based chemotaxis for Bacillus subtilis|