RNA

Part:BBa_K1363603

Designed by: Hanjia Tang   Group: iGEM14_USTC-China   (2014-09-29)

gate of no of RNA logic gates

the gate is a ribozyme whose activity is repressed when combined with key-of-no

The ribozyme is activated while the short RNA, key of no is no added. When lac is added, the short RNA combines with the ribozyme and restrains the cleavage, thus the level of GFP increases.

Fig.8 The principle of NO gate.
Fig.9 The schematic of NO gate.
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 129
    Illegal SpeI site found at 154
    Illegal PstI site found at 168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 154
    Illegal PstI site found at 168
    Illegal NotI site found at 161
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 154
    Illegal XbaI site found at 129
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 129
    Illegal SpeI site found at 154
    Illegal PstI site found at 168
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None