Protein_Domain
CWE

Part:BBa_K1362433

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

CWE (extein)

This sequence is coding for the amino acid sequence CWE. CWE is an improved non-native C-extein of the intein NpuDnaE. The associated N-extein is RGK (BBa_K1362434). Information about inteins is provided here: BBa_K1362400.

Exteins are the amino acids next to an intein. They have a great impact on splicing efficiency. Thus, good exteins are usually added between the protein of interest and inteins. However, they will remain as scars after splicing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None