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Part:BBa_K1362174

Designed by: Jan Gleixner   Group: iGEM14_Heidelberg   (2014-10-10)

Split sfGFP reconstitution N-terminal non-splicing control half

This part is the N-terminal non-splicing control half in a split sfGFP intein splicing assay. The corresponding C-terminal non-splicing control is BBa_K1362174, the N- and C-terminal splicing partners are BBa_K1362173 and BBa_K1362171, respectively. BBa_K1362170 served as positive control.

The assay was used to both characterize the reconstitution of split superfold GFP (BBa_I746916) as well as to demonstrate the splicing activity of the NpuDnaE split Intein BBa_K1362400. Visit the Heidelberg 2014's [http://2014.igem.org/Project wiki] for a detailed documentation or find a summary of our results in the experience section of this part.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 66


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