Composite

Part:BBa_K1362167

Designed by: Jan Gleixner   Group: iGEM14_Heidelberg   (2014-10-10)


RBS + Gp41-1 C-intein RFC[105] assembly construct with additional N-teminal insertion site



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 826
    Illegal AgeI site found at 938
    Illegal AgeI site found at 2026
    Illegal AgeI site found at 2138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1115
    Illegal BsaI site found at 2315
    Illegal BsaI.rc site found at 40
    Illegal BsaI.rc site found at 1240
    Illegal SapI.rc site found at 1155


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