Composite

Part:BBa_K1362109

Designed by: Jan Gleixner   Group: iGEM14_Heidelberg   (2014-10-09)


RBS + NpuDnaE N&C-Intein coexpression RFC[105] nonsplicing assembly construct (with His6)

This constructs allows for the easy assembly of parts for the co-expression of non-splicing His6-NpuDnaE(N)*-POI and POI-NpuDnaE(C)*-His6 proteins. Thereby it provides a fast way of establishing a negative control for splicing experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 826
    Illegal AgeI site found at 938
    Illegal AgeI site found at 2430
    Illegal AgeI site found at 2542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1115
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 40
    Illegal BsaI.rc site found at 1644


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Categories
Parameters
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