Composite

Part:BBa_K1362108

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)


RBS + NpuDnaE N&C-intein coexpression RFC[105] assembly construct (with His6)



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 826
    Illegal AgeI site found at 938
    Illegal AgeI site found at 2430
    Illegal AgeI site found at 2542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1115
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 40
    Illegal BsaI.rc site found at 1644


[edit]
Categories
Parameters
None