Composite
1NpuE(N*)

Part:BBa_K1362102

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)

This NpuDnaE(N*) intein assembly construct can serve as a control for the splicing reaction. The N-terminal cystein residue was mutated to a glycine to prevent the nucleophilic attack which initiates protein splicing. Read more about the intein assembly construct in BBa_K1362100. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 826
    Illegal AgeI site found at 938
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1115
    Illegal BsaI.rc site found at 40


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Parameters
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