Plasmid_Backbone
pSB1A30

Part:BBa_K1362091

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)


pSB1A30: High copy BioBrick cloning/expression backbone carrying Amp resitance



Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2185
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2191
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2185
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2185
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2185
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2200
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1173
    Illegal EcoRI site found at 2185
    Illegal XbaI site found at 2200
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//chassis/prokaryote/ecoli
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/inducible
//plasmidbackbone/version/3
//regulation/multiple
Parameters
chassis
copies100-300
insertNone
mcsBioBrick
negative_regulatorslacI Repressor
originpMB1
positive_regulatorsT7 RNAP, IPTG
resistanceA