Plasmid_Backbone
pSB1A30
Part:BBa_K1362091
Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha Group: iGEM14_Heidelberg (2014-10-08)
pSB1A30: High copy BioBrick cloning/expression backbone carrying Amp resitance
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2185
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2191 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2185 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2185
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2185
Plasmid lacks a suffix.
Illegal XbaI site found at 2200
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1173
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/inducible
//plasmidbackbone/version/3
//regulation/multiple
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/inducible
//plasmidbackbone/version/3
//regulation/multiple
| chassis | |
| copies | 100-300 |
| insert | None |
| mcs | BioBrick |
| negative_regulators | lacI Repressor |
| origin | pMB1 |
| positive_regulators | T7 RNAP, IPTG |
| resistance | A |