Composite

Part:BBa_K1362055

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

MinD MTS as RFC[105] C-terminal insert

The MinD MTS (membrane targeting sequence) from B. subtilis, when fused to the C-terminus of a protein, localizes it to the cytoplasmic face of the inner cell membrane in E. coli. This part functions as an RFC[105] insert. It consists of (BBa_K1362255) inserted into the carrier (BBa_K1362052).

Usage and Biology

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 95
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 106
    Illegal SapI.rc site found at 44


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Categories
Parameters
None