Composite

Part:BBa_K1362054

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

SsrA degradation tag as RFC[105] C-terminal insert

The SsrA tag directs the tagged protein to the ClpXP protease, causing degradation of this protein. This is a C-terminal tag. This part functions as an RFC[i] insert. It consists of (BBa_M0050) inserted into the carrier (BBa_K1362052).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 67


[edit]
Categories
Parameters
None