Composite

Part:BBa_K1362005

Designed by: Silvan Schmitz   Group: iGEM14_Heidelberg   (2014-10-09)


Sortase A non-circularization construct (with Smt3 and His6)

TODO

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 99
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 205
    Illegal BamHI site found at 396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1209
    Illegal AgeI site found at 1321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1498
    Illegal BsaI.rc site found at 424


[edit]
Categories
Parameters
None