Composite
CircSortA*

Part:BBa_K1362003

Designed by: Silvan Schmitz   Group: iGEM14_Heidelberg   (2014-10-09)

Sortase A circularization construct (with Smt3 and His6)

For more information see BBa_K1362002. Addition of BBa_K1362429 might improve expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 136
    Illegal BamHI site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1140
    Illegal AgeI site found at 1252
    Illegal AgeI site found at 1445
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1429
    Illegal BsaI.rc site found at 355


[edit]
Categories
Parameters
None