Composite
CircSortA

Part:BBa_K1362002

Designed by: Silvan Schmitz   Group: iGEM14_Heidelberg   (2014-10-09)

Sortase A circularization construct (with His6)

This part can be used to circularize any linear protein. The mRFP selection marker BBa_J04450 must be replaced by the protein of interest. After expression, the protein has to be purified and treated with TEV protease and Sortase A. Please visit our wiki for detailed [http://2014.igem.org/Team:Heidelberg/Toolbox/Circularization_Constructs information] and [http://2014.igem.org/Team:Heidelberg/Toolbox_Guide instructions].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 855
    Illegal AgeI site found at 967
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1144
    Illegal BsaI.rc site found at 70


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Categories
Parameters
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