Composite

Part:BBa_K136066

Designed by: Benoît D'hayer   Group: iGEM08_Paris   (2008-10-28)

araC/pBad - EnvZ*

inducible expression of EnvZ* by the araC/pBad. EnvZ is a transmembrane histidine kinase that, in response to osmotic changes, phosphorylates OmpR the natural inhibitor of flhDC promotor the master regulator of the flagella synthesis. EnvZ* is here a mutated version which constitutively phosphorylates OmpR.

Motility.PNG

Motility assay in a MG1655 (envZ-) strain. From left to right: no plasmid, pSB3K3-BBa_K136066 + 1% glucose, pSB3K3-BBa_K136066 + 0.2% arabinose Also the pBAD is quite leaky, there is a clear difference between it's activation and it's repression which shows the correct expression of EnvZ through it's action on motility.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1532
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1532
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1532
    Illegal BglII site found at 1452
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1532
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1532
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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