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Experience: None
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Regulatory

Part:BBa_K136004

Designed by: Cyprien Maisonnier   Group: iGEM08_Paris   (2008-08-12)


Promoter of flhDC gene from E. coli flagella

flhDC is the only class 1 gene that is part of the construction of the flagella. It is the master regulator of the biosynthesis of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).

The promoter of flhDC is regulated by many transcription factors, for instance, OmpR* and EnvZ* are two repressors of this gene.

Work in progress : Characterization of the repression by OmpR* and EnvZ*

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//regulation/multiple
Parameters
controlOmpR* & EnvZ*
directionForward
negative_regulators2
o_h
o_l
positive_regulators