Regulatory
Part:BBa_K136000
Designed by: Cyprien Maisonnier Group: iGEM08_Paris (2008-08-12)
Promoter of fliL gene from E. coli flagella
fliL is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ).
Its promoter is consitutively repressed.
It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).
Work in progress : Deeper quantitative characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
control | flhDC & fliA |
direction | Forward |
negative_regulators | |
o_h | |
o_l | |
positive_regulators | 1 |