Part:BBa_K1357010
eRFP reporter
This part consists of an RBS, the red chromogenic/fluorescent protein, mRFP, and a double terminator. This part is intended to serve as a useful reporter to determine what level of expression the system in which it is incorporated into without the need for a fluorescent microscope.
Usage and Biology
In order to show that a protein encoded on a plasmid will be expressed under desired conditions, a chromogenic change can be observed using this part. Instead of going through the process of using a fluorescent protein that could be costly to visualize for teams with less resources available or in the case that the given fluorescent protein interferes with your desired protein, a team can simply clone this behind their desired to determine expression levels of their desired protein. Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 573
Illegal AgeI site found at 685 - 1000COMPATIBLE WITH RFC[1000]
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