Device

Part:BBa_K1357004

Designed by: Matthew Tucker   Group: iGEM14_Virginia   (2014-10-06)

Constitutive NhaR Expression Device

This construct allows for the constitutive overexpression of the NhaR transcriptional activator. NhaR affects the transcription of the pgaABCD operon which is required for the production of biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamine (PGA). Overexpression of NhaR has been shown to increase biofilm formation in E. coli. This part is intended for use in projects aiming to use or explore biofilm formation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 236


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Categories
Parameters
None