Device

Part:BBa_K1357003

Designed by: Matthew Tucker   Group: iGEM14_Virginia   (2014-10-06)

IPTG Inducible NhaR Expression Construct

This construct allows for the controlled overexpression of the NhaR transcriptional activator. NhaR affects the transcription of the pgaABCD operon which is required for the production of biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamine (PGA). This part controls overexpression by utilizing an IPTG inducible promoter. Overexpression of NhaR has been shown to increase biofilm formation in E. coli. This part is intended for use in projects aiming to use or explore biofilm formation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 256


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Categories
Parameters
None