Coding

Part:BBa_K1357002

Designed by: Matthew Tucker   Group: iGEM14_Virginia   (2014-10-06)

NhaR Transcriptional Regulator

NhaR acts as a transcriptional activator in E. coli. It affects the transcription of the pgaABCD operon which is required for the production of biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamine (PGA). When over-expressed, NhaR is capable of increasing biofilm formation in E. coli. This part is intended to help teams seeking to increase biofilm formation in E. coli in their projects.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 175


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Categories
Parameters
None