Part:BBa_K1356002
Dispersin B gene with DsbA signalling sequence for export from N. multiformis
Dispersin B is a hydrolase protein that aids in the degradation of biofilms. This part contains the Dispersin B gene from part BBa_K1195000, with a signal sequence attached for export from the bacteria Nitrosospira Multiformis for the purpose of aiding in the breakup of biofilms in waste water management facilities.
Usage and Biology
A biofilm is a complex matrix composed of extracellular polymeric substances (EPS) and bacterial cells. They are generated as a defense mechanism by certain bacterial species. Although researchers have had difficulty isolating and identifying specific components of various biofilms it is generally accepted that biofilms are composed of: polysaccharides, proteins and extracellular DNA.
Dispersin B is an enzyme that targets polysaccharides in the matrix. It specifically targets "the glycosidic linkages of poly-β-1,6-GlcNAc" for hydrolysis (Itoh,Hinnebusch, Preston, & Romeo, 2005).
N. multiformis is an amonia-oxidizing bacterium common to waste-water treatment facilities. Using NCBI we blasted it's genome for a signaling sequence found on the DsbA protein, common to E. Coli., and were able to find a match. The sequence will enable Dispersin to be exported from N. multiformis and act to degrade problematic biofilm in the reactors.
(Ribbon image of Dispersin B)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1051
- 1000COMPATIBLE WITH RFC[1000]
References
Itoh, Y., Wang, X., Hinnebusch, B. J., Preston, J. F., & Romeo, T. (2005). Depolymerization of -1,6-N-Acetyl-D-Glucosamine Disrupts the Integrity of Diverse Bacterial Biofilms. Journal of Bacteriology. doi:10.1128/JB.187.1.382-387.2005
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