Part:BBa_K1356001
LMD-9 CRISPR 3 System
This is the Type II CRISPR3 system taken from Streptococcus thermophilus LMD-9. It is the Cas9, Csn1, Cas1, and Cas2 proteins along with the tracrRNA but . It may be used with a novel spacer/repeat region to target bacteriophage, plasmids, or any other form of incoming DNA. Cas9 is an endonuclease/exonuclease type protein and is the agent that inactivates incoming DNA. Csn1, Cas1, and Cas2 are involved in additional spacer acquisition, though the method is unknown. Cas9 can be directed by specially designed spacers or by spacers acquired by the CRISPR. The adaptive nature of the CRISPR3 makes it useful as an adaptive immune system for bacteria. It has been shown to be effective in a diverse range of microbes and can be used in any microbe. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3202
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