Coding
Terminal D

Part:BBa_K1354002

Designed by: Hua Joe Fung, Jiwoon Park, Alexa Orrico, Wilfrido Castillo   Group: iGEM14_Cooper_Union   (2014-10-03)

Bovine Terminal Deoxynucleotidyl Transferase: codon optimized for expression in E. coli

This sequence codes for bovine terminal deoxynucleotidyl transferase. This is an enzyme that is commonly found in mammalian immune cells. Natively, it aids in antibody gene recombination. It is known to polymerize deoxynucleotides onto the 3' end of single stranded oligonucleotides without the need for a template strand. This sequence has been codon optimized for E. coli. An Nde1 restriction site has been added to the 5' end of the sequence so that it can be cloned into the expression vector pET28b+.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 933


[edit]
Categories
Parameters
None