RBS
Part:BBa_K1351028
Designed by: Florian Riemer Group: iGEM14_LMU-Munich (2014-10-06)
LMU Bacillus RBS collection 1
This part was generated in RFC10, and has the following prefix and suffix:
Prefix Suffix 5' - GAATTC GCGGCCGC T TCTAGA G aaggagggata T ACTAGT A GCGGCCG CTGCAG - 3' EcoRI NotI XbaI SpeI NotI PstI
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:
Number | BBa_K1310 .. | Sequence |
---|---|---|
1 | 28 | aaggagggata |
2 | 29 | agaggaggata |
3 | 30 | aaggagagata |
4 | 31 | aggagaggata |
5 | 32 | aagaggagata |
6 | 33 | agaaagggata |
7 | 34 | aagaagagata |
The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/bsubtilis
//rbs/prokaryote/constitutive
//rbs/prokaryote/constitutive
None |