Regulatory

Part:BBa_K1351018

Designed by: Philipp Popp & Roman Herzog   Group: iGEM14_LMU-Munich   (2014-09-15)


This part was generated with the RFC10 standard and has the following prefix and suffix:

prefix with EcoRI, NotI and XbaI: GAATTCGCGGCCGCTTCTAGAG
suffix with SpeI, NotI and PstI: TACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


The Promoter regulates the sdpI (immunity against the cannibalism toxin sdpC) and the regulator sdpR in Bacillus subtilis. The overhangs were designed according to RFC10.
Primer fwd.: GAATTCGCGGCCGCTTCTAGAG gggttgtttttaaaaaaattcaagttataatg
Primer rev.: CTGCAGCGGCCGCTACTAGTA aaaaacatttagacatttagatatttgtataaatg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//regulation
Parameters
None