DNA

Part:BBa_K1347002

Designed by: Tori Speicher   Group: iGEM14_Nevada   (2014-09-23)

Jaz6

Jaz6 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with Jaz6; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1.


Usage and Biology

The 2014 Nevada iGEM team cloned Jaz6 fused to GFP under the control of a yeast pTEF promoter. This construct was ligated into a 2u plasmid and transformed into W303 yeast containing COI1 (integrated into the genome). Coronatine induced degradation of Jaz6-GFP was analyzed and the results are shown below.

Cells were incubated with coronatine for two hours and degradation was observed via western blot with an antibody for GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 181
    Illegal SapI.rc site found at 563


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