DNA

Part:BBa_K1347000

Designed by: Tori Speicher   Group: iGEM14_Nevada   (2014-09-21)

Jaz1

Jaz1 is a degron tag utilized in the coronatine pathway, found in plants. The protein of interest is tagged with Jaz1; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1.

The 2014 Nevada iGEM team submitted this part to the registry and used its homolog (Jaz6, BBa_K1347002) in coronatine inducible protein degradation studies. Shown below is how the Jaz degron, COI1 (E3 ligase) and small molecule inducers would be expected to interact in yeast cells. Jaz1 and coronatine should interact only in the presence of coronatine, not in the presence of auxin - a related hormone from plants.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 123
    Illegal BglII site found at 346
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 614


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Categories
Parameters
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