Regulatory

Part:BBa_K1346002

Designed by: Eleanor Amidei   Group: iGEM14_UCSF_UCB   (2014-07-15)

pTEF1(m7) - constitutive promoter

A mutant of the pTEF1 constitutive promoter. Seemingly the weakest out of the 5 that the UCSF/UCB team characterized.

140630 pTEF1 GFP ROUND 1.png

All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169


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