Part:BBa_K1343003

plac luxR double terminator

This part codes for LuxR under the Plac promoter. IPTG can induce the promoter to create the LuxR protein. LuxR can then form a complex with AHL and activate the Plux promoter.

In a characterization experiment, varying concentrations of 3o6c-AHL (3-oxohexanoyl-homoserine lactone) was added to a culture of E. coli K-12 Top 10 containing two different plasmids: pSB1C3-Plac-LuxR and pSB1A2-Plux-GFP. By adding the inducer IPTG to the bacteria LuxR was formed and addition of AHL to the bacteria enabled the complex LuxR-AHL to form. Binding of the complex activated the Plux promoter and GFP was expressed. There was a rise in the GFP fluorescence according to the rise in AHL concentrations until 0.1 µM AHL. At higher concentrations of AHL, saturation in the GFP expression was seen.

For the full experiment results see: http://2014.igem.org/Team:Technion-Israel/Experiments#gate2

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]