Part:BBa_K1342002

AspA LVA(-)

BBa_C0083 encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases. We removed LVA tag in BBa_C0083.
AspA is coding sequence of aspartase carries out reversible conversion of L-aspartate to fumarate and ammonia.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 72
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 964
  Illegal AgeI site found at 493
  Illegal AgeI site found at 1345
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1004
  

TLC Assay

fig.1
Analysis of aspartic acid synthesized by E. coli JM109/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, 2 no addition into culture (diluted 1:50 and 1:100 by synthesis medium, respectively) 3, additon of cadmium ion (250μM) 4, 5, 6 (diluted 1:50, 1:100 and 1:200 by synthesis medium, respectively)

fig.2
Estimation of aspartic acid content in spot Each intensity of spots indicating the content of aspartic acid, was estimated by Image J. .