Part:BBa_K1341020

Improved by AHUT_China 2016

Solution24578 is the shortest pathway in the project of 2014 of AHUT-China, which Node NO.5 is a necessary node to be passed in order to get to Node NO.8. However, in our project of this year, we don’t have to pass Site NO.5 while taking Site NO.2 as the start point and Site NO.8 as the terminal, which provides us with more diverse pathway choices and more conforms to the real situation. In our project of 2016, the DNA single strands of the sites and lines are connected by bridging-PCR. 12 information bits are added to the DNA single strand of the lines, which the pre-4bp sequences contain the information of identification point, the mid-4bp sequences contain the information of the specified line, and the post-4bp contain the information of the path length. In order to obtain Solution24578, it needs to construct recombinant plasmid in every possible pathway. And then the recombinant plasmid was inserted in E.coli, where the fluorescent protein gene in Node NO.5 get expressed. Thus, all the pathways passing Node NO.5 screened out. By contrast, we don’t have to go through a series of complex process like what have been depicted above in order to obtain the shortest pathway <2-6-8> in this year.