Composite

Part:BBa_K1339024

Designed by: Madoka NAGATA   Group: iGEM14_Tokyo-NoKoGen   (2014-10-02)

Pconst(low)-RBS-G3DH-T0 terminator


This part contains the ORF of G3DH from Rhizobium tumefaciens EHA101, which converts glucose to 3-ketoglucose following the RBS. A double terminator is located on the downstream of G3DH. This G3DH is located on the downstream of constitutive promoter. Because G3DH shows wide substrate specificity, G3DH can also convert trehalose (a disaccharide made up of two glucose moieties) into 3,3´-diketotrehalose, which is a trehalase inhibitor.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2409
    Illegal NotI site found at 390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 630
    Illegal NgoMIV site found at 1846
    Illegal NgoMIV site found at 2142
    Illegal NgoMIV site found at 2695
    Illegal NgoMIV site found at 2760
    Illegal AgeI site found at 812
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1903
    Illegal SapI site found at 2007


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