Composite

Part:BBa_K1339016

Designed by: Madoka NAGATA   Group: iGEM14_Tokyo-NoKoGen   (2014-10-02)

Pconst(medium)-RBS-otsB-RBS-otsA-dT
This BioBrick part is constructed by adding constitutive promoter(BBa_J23106) to our construct BBa_K1339004.
To confirm the production of trehalose, we cultured the transformed E.coli and confirmed by thin layer chromatography(TLC).
The figure below is our result.
Noko14_Trehaloseproductiontlc.png
The spot of a transformant harboring otsBA under constitutive promoter Pmedium was almost the same height as the standard sample of trehalose.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 516
    Illegal BamHI site found at 1271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aPconst(medium)-RBS-otsB-RBS-otsA-dT