Composite

Part:BBa_K1339013

Designed by: Madoka NAGATA   Group: iGEM14_Tokyo-NoKoGen   (2014-10-02)

Pbad-RBS-otsB-RBS-otsA-dT

Promoter(PBAD)-RBS-otsB-RBS-otsA-dT
This BioBrick part is constructed by adding arabinose inducible promoter(BBa_I0500) to our construct BBa_K1339004.

This part contains the Escherichia coli K-12 otsB and otsA genes under the transcriptional control of the pBAD promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1691
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2446
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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